HAO1 antibody (HRP)

Catalog No. ABIN7565881

This Rabbit Polyclonal antibody specifically detects HAO1 in WB, IHC and ELISA. It exhibits reactivity toward Aspergillus niger.

Quick Overview for HAO1 antibody (HRP) (ABIN7565881)

Target: HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))

Reactivity: Aspergillus niger

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAO1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HAO1 Antibody (HRP)

Purpose: Glucose Oxidase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Characteristics: Synonyms: rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Purification: Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Glucose Oxidase [Aspergillus niger]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Glucose Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in Immunohistochemistry. Optimal titers should be obtained by researcher. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:5,000 - 1:25,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for HAO1

Target: HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))

Alternative Name: gox

Background: Background: Glucose Oxidase is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites, used for the determination of free glucose in body fluids, and has many applications in biotechnologies.

UniProt: P13006

Pathways: Monocarboxylic Acid Catabolic Process