Serum antibody (HRP)

Catalog No. ABIN7565887

This Rabbit Polyclonal antibody specifically detects Serum in ELISA, WB, IHC and DB. It exhibits reactivity toward Guinea Pig.

Quick Overview for Serum antibody (HRP) (ABIN7565887)

Target: Serum

Reactivity: Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Product Details

Purpose: Guinea Pig Serum Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum.

Characteristics: Synonyms: Rabbit anti-Guinea Pig IgG Peroxidase Conjugated Antibody, Rabbit anti-Guinea Pig IgG HRP Conjugated Antibody, Rabbit anti-Guinea Pig IgG Peroxidase Conjugated Antibody, Rabbit anti-Guinea Pig IgG HRP Conjugated Antibody, Rabbit anti-Guinea Pig serum Peroxidase Conjugated Antibody, Rabbit anti-Guinea Pig serum HRP Conjugated Antibody

Purification: Guinea Pig Serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Guinea Pig serum proteins

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Guinea Pig Serum Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized ELISA Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Serum

Background: Background: Anti-Guinea Pig Peroxidase antibody detects guinea pig serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Guinea Pig Serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.