Streptavidin antibody (Biotin)

Catalog No. ABIN7565911

This Rabbit Polyclonal antibody specifically detects Streptavidin in ELISA and DB. It exhibits reactivity toward Streptomyces avidinii.

Quick Overview for Streptavidin antibody (Biotin) (ABIN7565911)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is conjugated to Biotin

Application: ELISA, Dot Blot (DB)

Product Details anti-Streptavidin Antibody (Biotin)

Purpose: Streptavidin Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin.

Characteristics: Synonyms: rabbit anti-Streptavidin Antibody biotin Conjugation, biotin conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin BAC Antibody

Purification: Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Streptavidin (Streptomyces avidinii)

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher. ELISA Dilution: 1:2,000-1:20,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Streptavidin

Target: Streptavidin

Background: Background: Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10-14 Mol/l to 10-15 Mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin through the antibody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interaction.