GAPDH antibody

Catalog No. ABIN7565915

The Mouse Monoclonal anti-GAPDH antibody is suitable to detect GAPDH in samples from Rabbit. It has been validated for WB, ELISA, IHC, IP, FM and MA.

Quick Overview for GAPDH antibody (ABIN7565915)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: 33F2-C8-G6-E3

Product Details anti-GAPDH Antibody

Purpose: GAPDH Antibody

Cross-Reactivity (Details): Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase.

Characteristics: Synonyms: mouse anti-GAPDH antibody, Loading Control Antibody, G3PDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

Purification: The antibody is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein.

Immunogen Type: Native Protein

Isotype: IgM

Application Details

Application Notes: Application Note: Anti-GAPDH (Mouse) has been tested in ELISA and western blot. This product is suitable in IHC and IF. Specific conditions should be optimized by the end user. Expect a band of ~38 kDa in size corresponding to Glyceraldehyde 3-Phosphate Dehydrogenase protein by Western blot in the appropriate cell lysate or extract. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Background: GAPDH Loading Control Monoclonal Antibody detects Glyceraldehyde-3-Phosphate Dehydrogenase. GAPDH is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. Anti-GAPDH antibody is widely used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue and is ideal for Neuroscience, Cell Signaling and Cancer Research.

Gene ID: 100009074

NCBI Accession: NP_001075722

UniProt: P46406