GAPDH antibody (N-Term)

Catalog No. ABIN7565921

The Rabbit Polyclonal anti-GAPDH antibody is suitable to detect GAPDH in samples from Human. It has been validated for WB and ELISA.

Quick Overview for GAPDH antibody (N-Term) (ABIN7565921)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GAPDH Antibody

Binding Specificity: N-Term

Purpose: GAPDH Antibody

Cross-Reactivity (Details): Anti-GAPDH Antibody is directed against human GAPDH protein.

Characteristics: Synonyms: rabbit anti-GAPDH antibody, rabbit anti-Glyceraldehyde-3-phosphate dehydrogenase antibody, GAPDH Loading Control Antibody, Anti-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-GAPDH Antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract.  GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. This Anti-GAPDH Antibody product is a new product replacing p/n 600-401-A33. Western Blot Dilution: 1:1000 ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.17 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-GAPDH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Background: GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. A loading control antibody is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience.

Gene ID: 2597, 7669492

UniProt: P04406