MUC4 antibody

Catalog No. ABIN7565926

This Rabbit Polyclonal antibody specifically detects MUC4 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for MUC4 antibody (ABIN7565926)

Target: MUC4 (Mucin 4, Cell Surface Associated (MUC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MUC4 Antibody

Purpose: MUC4 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human MUC4 protein.

Characteristics: Synonyms: Rabbit Anti-MUC4 Antibody, Rabbit Anti-Mucin 4 Antibody, MUC4, Mucin-4, Ascites sialoglycoprotein, ASGP, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, Mucin-4 alpha chain, Ascites sialoglycoprotein 1, ASGP-1, Mucin-4 beta chain, Ascites sialoglycoprotein 2, ASGP-2, MUC

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human Mucin-4.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-MUC4 Antibody was tested in ELISA, Western Blot, and Immunohistochemistry. Antibodies shows no cross reactivity to non-mucilated proteins. Positive control used in WB were HPAC, HPAF-II, and Capan-2 cell lines and IHC was HPAC cell line. Immunohistochemistry Dilution: 1:1000 Western Blot Dilution: 1:1000 ELISA Dilution: 5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for MUC4

Target: MUC4 (Mucin 4, Cell Surface Associated (MUC4))

Alternative Name: MUC4

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). MUC4 (Mucin 4, Cell Surface Associated) is a Protein Coding gene. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. Highly glycosylated proteins called mucins, are the major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix. MUC4's ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. MUC4 seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. These glycoproteins play an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 Tyr-1248. In polarized epithelial cells segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a co-receptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. May play a role in tumor progression. MUC4 is associated with diseases such as bile duct cancer, keratitis, adenosquamous carcinoma, and pancreatic adenocarcinomas. Anti-MUC4 Antibody is useful for researchers interested in digestion, cancer research, and extracellular matrix Antibodies.

Gene ID: 4585

NCBI Accession: NP_004523

UniProt: Q99102

Pathways: Regulation of Leukocyte Mediated Immunity