GATA1 antibody

Catalog No. ABIN7565929

This Rabbit Polyclonal antibody specifically detects GATA1 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for GATA1 antibody (ABIN7565929)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GATA1 Antibody

Purpose: Gata1 Antibody

Cross-Reactivity (Details): Anti-Gata1 is directed against mouse Gata1.

Characteristics: Synonyms: rabbit anti-Gata1 antibody, Gata-1, gata 1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, GF-1, NF-E1 DNA-binding protein, Gf-1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-Gata1 Antibody has been tested by ELISA and Western Blot. Expect a band approximately 43 kDa in size corresponding to mouse Gata1 by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:15,000 - 1:75,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.73 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: Gata1

Background: Background: Gata1, also know as erythroid transcription factor, is critical in red blood cell and platelet structure and development. It regulates the switch from fetal hemoglobulin to adult hemoglobulin. Anti-Gata1 antibody is ideal for researchers interested in cell growth and cancer research.

Gene ID: 14460

NCBI Accession: NP_032115

UniProt: P17679

Pathways: Cellular Response to Molecule of Bacterial Origin