NRF2 antibody (C-Term)

Catalog No. ABIN7565937

This anti-NRF2 antibody is a Rabbit Polyclonal antibody detecting NRF2 in WB and ELISA. Suitable for Mouse.

Quick Overview for NRF2 antibody (C-Term) (ABIN7565937)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NRF2 Antibody

Binding Specificity: C-Term

Purpose: Nrf2 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against mouse Nrf2 protein.

Characteristics: Synonyms: rabbit anti-Nrf2 antibody, Nrf 2, Nrf-2 Antibody, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, Nfe2l2, HEBP1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-NRF-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding near the c-term region.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: This affinity purified Nrf2 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66.9 kDa in size corresponding to Nrf-2 proteins by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: Nfe2l2

Background: Background: Anti-Nrf-2 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Nrf2 links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Nrf2 is localized in the cytoplasm and nucleus and ubiquitously expressed with the highest concentrations in the kidney, muscle, lung, heart, liver, and brain. Anti-Nrf2 antibody is useful for researchers interested in cellular growth, transcription factor, and cancer research.

Gene ID: 18024

NCBI Accession: NP_035032

UniProt: Q60795

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling