SETDB2 antibody

Catalog No. ABIN7565943

This anti-SETDB2 antibody is a Rabbit Polyclonal antibody detecting SETDB2 in WB and ELISA. Suitable for Mouse.

Quick Overview for SETDB2 antibody (ABIN7565943)

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SETDB2 Antibody

Purpose: Setdb2 Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against mouse Setdb2.

Characteristics: Synonyms: rabbit anti-Setdb2 Antibody, Histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, Setdb 2 antibody, Gm293

Purification: The product was purified from monospecific antiserum by Protein A affinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Setdb2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length protein corresponding to mouse Setdb2.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: Anti-Setdb2 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80.6 kDa in size corresponding to Setdb2 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:10,000 - 1:100,000 ELISA Dilution: 1:2,500 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SETDB2

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Alternative Name: Setdb2

Background: Background: Setdb2 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). Setdb2 and the neighboring gene Phf11 were linked to familial atopy/ asthma and are in a "critical region" commonly deleted in CLL. Setdb2 is a candidate histone methyltransferase as a member of the Su(var) 3-9 family of SET domain-containing enzymes. Other predicted domains in Setdb2 include a methylcytosine binding domain and a large bifurcation domain interrupting the putative histone methyltransferase (SET) domain whose function is unknown. Anti-Setdb2 antibody is ideal for researchers interested in Cancer Research and Epigenetics Research.