EFNB1/EFNB2/EFNB3 (C-Term) antibody

Catalog No. ABIN7565950

This Rabbit Polyclonal antibody specifically detects EFNB1/EFNB2/EFNB3 in ELISA, FM, IF and WB. It exhibits reactivity toward Human.

Quick Overview for EFNB1/EFNB2/EFNB3 (C-Term) antibody (ABIN7565950)

Target: EFNB1/EFNB2/EFNB3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Fluorescence Microscopy (FM), Immunofluorescence (IF), Western Blotting (WB)

Product Details

Binding Specificity: C-Term

Purpose: Ephrin B1, B2, B3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Ephrin B1, B2, B3.

Characteristics: Synonyms: Rabbit Anti-Ephrin B1, B2, B3 Antibody, Ephrin-B1, Ephrin-B2, Ephrin-B3, ELK Ligand, EPLG2, LERK2, EFL3, Craniofrontonasal Syndrome (Craniofrontonasal Dysplasia), EPH-Related Receptor Transmembrane Ligand ELK-L3, Eph-Related Receptor Tyrosine Kinase Ligand 2, Eph-Related Receptor Tyrosine Kinase Ligand 8, EPH-Related Receptor Tyrosine Kinase Ligand 2, LERK-2, LERK-8, Elk-L, EFL-3, ELK-L, CFND, EFL6, EFB1, CFNS

Purification: This affinity purified antibody is directed against human Ephrin B1, B2, B3.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Ephrin B1, B2, B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-Ephrin B1, B2, B3 Antibody has been tested in ELISA, Western Blot, and Immunofluorescence. Expect a band at ~35-38 kDa in western blot using appropriate lysates or tissues. Positive control used Xenopus laevis and mouse kidney in WB and Xenopus laevis in IF. Western Blot Dilution: 1:1000 ELISA Dilution: 1:10,000-50,000 IF Microscopy Dilution: 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.05 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: EFNB1/EFNB2/EFNB3

Alternative Name: EFNB1, Efnb2, EFNB3

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). Ephrin B1, B2, B3 are cell surface transmembrane ligands for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring. Ephrin B1 shows high affinity for the receptor tyrosine kinase EPHB1/ELK and can bind EPHB2 and EPHB3. Ephrin B3 may play a pivotal role in forebrain function. They binds to, and induces collapse of, commissural axons/growth cones in vitro. And may play a role in constraining the orientation of longitudinally projecting axons. Anti-Ephrin B1, B2, B3 Antibody is useful for researchers interested in Developmental Biology and Tyrosine Kinases/Adaptors.

Gene ID: 1947

NCBI Accession: NP_004420

UniProt: P52800