POLR2A/RPB1 antibody (N-Term)

Catalog No. ABIN7565953

This Rabbit Polyclonal antibody specifically detects POLR2A/RPB1 in WB, ELISA, ChIP and FM. It exhibits reactivity toward Human.

Quick Overview for POLR2A/RPB1 antibody (N-Term) (ABIN7565953)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: N-Term

Purpose: POL II Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human POL II.

Characteristics: Synonyms: RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A,

Purification: This affinity purified antibody is directed against human POL II.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5', NR4A1 3' and FOS 3'. The negative control was total rabbit IgG. ChIP Dilution: User Optimized Western Blot Dilution: 1:1000 ELISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A

Background: Background: Anti-POL II antibody will detect DNA-directed RNA polymerase II subunit RPB1, an enzyme composed of 12 subunits, which initiates the transcription of RNA from DNA, synthesizing many functional non-coding RNAs as well as messenger RNA precursors. POL II encodes a core element of the transcription machinery. Malfunctioning of the Pol II gene can cause diseases such as Cockayne Syndrome B and La Crosse Encephalitis. Anti-POL II Antibody is useful for researchers interested in DNA damage and repair studies.

Gene ID: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatory RNA Pathways