CCL3 antibody

Catalog No. ABIN7565958

This anti-CCL3 antibody is a Rabbit Polyclonal antibody detecting CCL3 in WB and ELISA. Suitable for Human.

Quick Overview for CCL3 antibody (ABIN7565958)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL3 Antibody

Purpose: MIP-1 alpha Antibody

Characteristics: Synonyms: Tonsillar lymphocyte LD78 alpha protein antibody, CCL 3 antibody, Chemokine CC Motif Ligand 3 antibody, G0/G1 switch regulatory protein 19 1 antibody, SIS beta antibody, Small inducible cytokine A3 antibody, rabbit anti-MIP-1 alpha Antibody

Purification: This antiserum has been heated to 56° C for 30 minutes.

Sterility: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human MIP-1a  produced in E.coli.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: This antiserum against Human MIP-1a has been tested for use in ELISA of human MIP-1a found in cell supernatants or in plasma or serum.  In ELISA formats and other immunoreactive assays, this antibody will recognize recombinant and native human MIP-1a present in body fluids and cell supernatants.  This antiserum has not been evaluated for its ability to stain human MIP-1a in tissue sections, nor for its ability to neutralize human MIP-1a in bioassays, nor for its performance in immunoblot analysis. Reactivity in other immunoassays is unknown. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: CCL3

Background: Background: C-C motif chemokine 3, CCL3 or (MIP-1a) is a monokine with inflammatory and chemokinetic properties. CCL3 binds to CCR1, CCR4 and CCR5. It is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Gene ID: 6348

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy