Pol (C-Term) antibody

Catalog No. ABIN7565974

This Rabbit Polyclonal antibody specifically detects Pol in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for Pol (C-Term) antibody (ABIN7565974)

Target: Pol

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: C-Term

Purpose: Orf2 Antibody

Cross-Reactivity (Details): Anti-Orf2 is directed against mouse ORF2 near a c-terminus position.

Characteristics: Synonyms: Rabbit Anti-Orf2 Antibody, LINE-1 retrotransposable element ORF2 protein, Endonuclease reverse transcriptase, Line1 protein, Orf2p, Retrovirus-related Pol polyprotein LINE-1, Long interspersed element-1

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-ORF2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminus of mouse ORF2 protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: This affinity purified antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~150 kDa in size corresponding to Orf2 by western blotting in the liver cell lysates or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000-1:150,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.07 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Pol

Target Type: Viral Protein

Background: Background: Anti-Orf2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). The multi-functional nuclear protein ORF2p is involved in reverse transcriptase and endonuclease processes. ORF2 elements are epigenetically silenced throughout embyronic development and it is suggested that mutations of L1 would result in detrimental de novo insertions into the genome, negatively affecting gene expression.  L1 elements are critical components in the mammalian genome, and much is still not known regarding these proteins. Studies implicate that line-1 is activated in fetal oocytes and  L1ORF2p may contribute to the eradication or selection of the oocytes.

Gene ID: 2731767