PKM antibody

Catalog No. ABIN7565975

The Rabbit Polyclonal anti-PKM antibody has been validated for ELISA and WB. It is suitable to detect PKM in samples from Human.

Quick Overview for PKM antibody (ABIN7565975)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-PKM Antibody

Purpose: PKM2 Antibody

Cross-Reactivity (Details): Anti-PKM2 is directed against human PKM2 at an internal position.

Characteristics: Synonyms: rabbit anti-PKM2 antibody, PKM 2, PKM-2, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, OIP3, OIP-3, PK2, PK3

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: This affinity purified antibody has been tested for use in western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band 56-58 kDa in size corresponding to PKM2 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM

Background: Background: Anti-PKM2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Pyruvate kinase has been shown to be expressed in tumor, embryonic and normal proliferating cells in varying levels. Studies show that when PKM2 is secreted it can induce EGFR phosphorylation which stimulates the EGFR downstream pathway. Previously PKM has been shown to be critical for aerobic glycolysis. Four isoforms of PKM play alternative roles within the system and post-translational modifications along these isoforms induce multiple pathways making this Anti-PKM2 antibody ideal for investigators involved in cancer research and post-translational modifications.

Gene ID: 5315

NCBI Accession: NP_001193725

UniProt: P14618

Pathways: Warburg Effect