RFP antibody

Catalog No. ABIN7565988

This anti-RFP antibody is a Rabbit Polyclonal antibody detecting RFP in WB, ELISA, IF, IHC, FACS and FM. Suitable for Discosoma.

Quick Overview for RFP antibody (ABIN7565988)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Product Details anti-RFP Antibody

Purpose: RFP Antibody Pre-adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Characteristics: Synonyms: rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Preadsorption: Pre-Adsorbed

Sterility: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: RFP Antibody Pre-adsorbed is designed for detecting RFP and its variants, tested across ELISA, Western blot, IF, IHC, and suitable for EM, FC, FISH, IP, and multiplex assays. It is applicable for ELISA in both sandwich and capture formats. Researchers should determine optimal titers for specific applications. Recommended starting dilutions are: ELISA 1:28,700 - 1:48,700, FC, IF, IHC 1:200 - 1:2,000, WB 1:1,000 - 1:5,000. Flow Cytometry Dilution: 1:200 - 1:2,000 Immunohistochemistry Dilution: 1:200-1:2,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:20,000 - 1:50,000 IF Microscopy Dilution: 1:200-1:2,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.05 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: DsRed

Background: Background: RFP Antibody Pre-adsorbed is a high-quality, rabbit polyclonal antibody specifically designed for the detection of red fluorescent protein (RFP) and its variants like mCherry, mScarlet, and tdTomato. This product is suitable for a broad range of applications including Western blot (WB), ELISA, immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and more. It is uniquely pre-adsorbed to minimize cross-reactivity, offering high specificity and sensitivity. With over 900 references, this product stands as a proven tool in the scientific community, supporting a wide range of research studies from cellular signaling to disease pathology.

UniProt: Q9U6Y8