KTI3 antibody (HRP)

Catalog No. ABIN7565993

The Rabbit Polyclonal anti-KTI3 antibody is suitable to detect KTI3 in samples from Lima bean. It has been validated for WB, DB and IP.

Quick Overview for KTI3 antibody (HRP) (ABIN7565993)

Target: KTI3

Reactivity: Lima bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), Dot Blot (DB), Immunoprecipitation (IP)

Product Details

Purpose: Trypsin Inhibitor Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Characteristics: Synonyms: rabbit anti-trypsin inhibitor antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Purification: Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Trypsin Inhibitor [Lima Bean]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Trypsin Inhibitor Peroxidase Conjugated Antibody has been tested by Dot blot and is suitable to be assayed against 1.0 μg of Trypsin Inhibitor [Lima Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Immunoprecipitation Dilution: 1:100 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: KTI3

Background: Background: Trypsin Inhibitors are chemicals that reduce the availability of biologically active trypsin, an enzyme essential to nutrition of many animals, including humans. Tumor Associated Trypsin Inhibitor (TATI) has been used as a marker for various carcinomas and useful in cancer diagnosis.

Gene ID: 547831

UniProt: P01070