XDH antibody (HRP)

Catalog No. ABIN7565995

This Rabbit Polyclonal antibody specifically detects XDH in WB, IP and DB. It exhibits reactivity toward Cow.

Quick Overview for XDH antibody (HRP) (ABIN7565995)

Target: XDH (Xanthine Dehydrogenase (XDH))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Product Details anti-XDH Antibody (HRP)

Purpose: Xanthine Oxidase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Characteristics: Synonyms: rabbit anti-Xanthine Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

Purification: Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Xanthine Oxidase [Bovine Buttermilk]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Xanthine Oxidase Peroxidase conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Immunoprecipitation Dilution: 1:100 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: XDH

Background: Background: Xanthine Oxidase is a key enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine, xanthine to uric acid, and contributes to the generation of reactive oxygen species.

Gene ID: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity