Red Blood Cell antibody (Magnetic Particles)

Catalog No. ABIN7566001

This Rabbit Polyclonal antibody specifically detects Red Blood Cell in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Red Blood Cell antibody (Magnetic Particles) (ABIN7566001)

Target: Red Blood Cell (RBC)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Red Blood Cell antibody is conjugated to Magnetic Particles

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Red Blood Cell Antibody (Magnetic Particles)

Purpose: Red Blood Cell Depletion Magnetic Beads

Characteristics: Synonyms: Red Blood Cell Depletion Magnetic Beads, Anti-RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti RBC, rabbit antibody to human red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrotyes, anti-erythrocytes antibody, magnetic bead, nanoparticles, paramagnetic, Magnetic bead-conjugated

Immunogen:

Immunogen: Human washed pooled Red Blood Cells (RBC)

Immunogen Type: Other

Application Details

Application Notes: Application Note: Red Blood Cell Depletion Magnetic Beads have been tested in binding assays for separation of Red Blood Cells, from whole blood. Remaining white blood cells can be used for downstream assays such as immunoassays, Western blots, FACS, and immunohistochemistry. For 100 μL of whole, fresh blood cells add 75 μL of Depletion beads, expecting 75 - 95 % depletion of RBC after one pass. Optimal amount for applications should be determined by the researcher. Flow Cytometry Dilution: User Optimized Western Blot Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Concentration: 20 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. DO NOT FREEZE. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Expiry Date: 12 months

Target Details for Red Blood Cell

Target: Red Blood Cell (RBC)

Alternative Name: RBC

Background: Background: Red Blood Cell Depletion Magnetic Beads recognizes human red blood cells. Red Blood Cell Depletion Magnetic Beads is recommended for the depletion of human Red Blood Cells in samples prior to downstream immunological and non-immunological assays and has been tested in Flow Cytometry. Some additional assays could include Colony Forming Unit (CFU) Assay and Immunohistochemistry among others. These magnetic beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The beads have a large surface area with high capture efficiencies. Bead mean diameter is ~0.5 μm, bead concentration is 20 mg/mL.