Recombinant N-Cadherin antibody

Catalog No. ABIN7566409

This Human Monoclonal antibody specifically detects N-Cadherin in WB, IHC, FACS and ELISA. It exhibits reactivity toward Human and Zebrafish (Danio rerio).

Quick Overview for Recombinant N-Cadherin antibody (ABIN7566409)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Zebrafish (Danio rerio)

Host: Human

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: SH1352-B11

Product Details anti-N-Cadherin Antibody

Purpose: anti-Cdh2, mAb (rec.) (SH1352-B11)

Characteristics:

Recombinant Antibody. Recognizes zebrafish and human Cdh2. Applications: ELISA, FACS, IHC, WB. Clone: SH1352-B11. Isotype: Human IgG1. Formulation: Liquid. In PBS. The cadherin superfamily of cell adhesion molecules regulate cell-cell interaction and are key factors in developmental morphogenesis and tissue repair. Cadherin-2 (N-cadherin, Cdh2), expressed in the vertebrate nervous system, is a type I transmembrane glycoprotein expressed in fusion to an N-terminal propeptide, necessary to avoid intracellular interaction of the molecule. Mature Cdh2 consists of five extracellular immunoglobulin (Ig)-like domains (Cdh2-ECDs), a single-pass transmembrane region and an intracellular domain for the interaction with the cell cytoskeleton, mainly mediated by beta-catenin binding. Throughout the early developmental stages of vertebrates, Cdh2 is expressed almost ubiquitously. Later stages its expression seems to be restricted to the central nervous system (CNS) in specific neuronal populations, but it also persists in cardiac muscle. In the central and peripheral nervous systems, Cdh2-dependent cell-cell interactions mediate a variety of neurodevelopmental processes including tissue architecture formation, neuronal migration, axon outgrowth, target recognition and synaptogenesis. Cdh2 levels are often increased during cancer invasion and metastatic behavior resulting in enhanced cell motility. For its role in tumor progression, Cdh2 has been considered a potential biomarker for the prediction of metastases and as an important prognostic factor. As a member of the cadherin superfamily of proteins, zebrafish Cdh2 (zCdh2) shares a high degree of amino acid homology with other members of this family. Zebrafish (Danio rerio) Cdh2-ECD has an overall amino acid identity of 63 % to zebrafish Cadherin-4-ECD, with a peak of 68 % in domains 1. The Ig-like structure of the 5 extracellular domains and the high sequence similarity to other cadherins requires a careful process of antibody generation against the zCdh2-ECD to avoid unwanted cross-reactivities and a thorough process of binding specificity characterization.

The cadherin superfamily of cell adhesion molecules regulate cell-cell interaction and are key factors in developmental morphogenesis and tissue repair. Cadherin-2 (N-cadherin, Cdh2), expressed in the vertebrate nervous system, is a type I transmembrane glycoprotein expressed in fusion to an N-terminal propeptide, necessary to avoid intracellular interaction of the molecule. Mature Cdh2 consists of five extracellular immunoglobulin (Ig)-like domains (Cdh2-ECDs), a single-pass transmembrane region and an intracellular domain for the interaction with the cell cytoskeleton, mainly mediated by beta-catenin binding. Throughout the early developmental stages of vertebrates, Cdh2 is expressed almost ubiquitously. Later stages its expression seems to be restricted to the central nervous system (CNS) in specific neuronal populations, but it also persists in cardiac muscle. In the central and peripheral nervous systems, Cdh2-dependent cell-cell interactions mediate a variety of neurodevelopmental processes including tissue architecture formation, neuronal migration, axon outgrowth, target recognition and synaptogenesis. Cdh2 levels are often increased during cancer invasion and metastatic behavior resulting in enhanced cell motility. For its role in tumor progression, Cdh2 has been considered a potential biomarker for the prediction of metastases and as an important prognostic factor. As a member of the cadherin superfamily of proteins, zebrafish Cdh2 (zCdh2) shares a high degree of amino acid homology with other members of this family. Zebrafish (Danio rerio) Cdh2-ECD has an overall amino acid identity of 63 % to zebrafish Cadherin-4-ECD, with a peak of 68 % in domains 1. The Ig-like structure of the 5 extracellular domains and the high sequence similarity to other cadherins requires a careful process of antibody generation against the zCdh2-ECD to avoid unwanted cross-reactivities and a thorough process of binding specificity characterization.

Purification: Puified

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant zebrafish Cdh2 domain 1 and domain 2 containing a C-terminal human Fc-tag.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.

Storage: 4 °C,-20 °C

Storage Comment:

Stable for at least 1 year after receipt when stored at -20°C.

Stable for at least 1 week when stored at +4°C.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: Cdh2

UniProt: Q90275

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane