Fibronectin antibody

Catalog No. ABIN7566419

The Mouse Monoclonal anti-Fibronectin antibody has been validated for WB, IHC, ELISA, ICC and Func. It is suitable to detect Fibronectin in samples from Human, Rat, Mouse, Pig, Cow, Chicken, Dog and Monkey.

Quick Overview for Fibronectin antibody (ABIN7566419)

Target: Fibronectin

Reactivity: Human, Rat, Mouse, Pig, Cow, Chicken, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Functional Studies (Func)

Clone: IST-9

Product Details anti-Fibronectin Antibody

Purpose: anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free)

Characteristics:

Monoclonal Antibody. Isotype: Mouse IgG1.Immunogen: Recombinant Extra Domain A (EDA) region of human cellular fibronectin.. Application: ELISA, FUNC (Blocking), ICC, IHC, WB. Sterile liquid. In PBS. Recombinant Extra Domain A (EDA) region of human cellular fibronectin. Fibronectin is a major component of the extracellular matrix that binds to integrins, cell surfaces and various compounds including collagen, fibrin, heparin, DNA and actin. It occurs in two main forms: plasma and cellular fibronectin. EDA (Extra Domain A)-containing cellular fibronectin (EDA-FN) plays a major role in cell adhesion, growth, migration and differentiation and it is important for processes such as wound healing and embryonic development. It is associated with fibroblast differentiation by increasing alpha-SMA expression, collagen deposition, cell contractility and focal adhesion kinase (FAK) activation. EDA-FN promotes cell spread by interacting with the integrins alpha5-beta1, alpha4-beta1 and alpha9-beta1 and has been shown to promote angiogenesis, lymphangiogenesis and metastasis of colorectal tumors. In transformed or tumor-derived cells the EDA segment is about 10-times higher than in fibronectin from normal fibroblasts and it may therefore be a significant marker for malignancy.

Fibronectin is a major component of the extracellular matrix that binds to integrins, cell surfaces and various compounds including collagen, fibrin, heparin, DNA and actin. It occurs in two main forms: plasma and cellular fibronectin. EDA (Extra Domain A)-containing cellular fibronectin (EDA-FN) plays a major role in cell adhesion, growth, migration and differentiation and it is important for processes such as wound healing and embryonic development. It is associated with fibroblast differentiation by increasing alpha-SMA expression, collagen deposition, cell contractility and focal adhesion kinase (FAK) activation. EDA-FN promotes cell spread by interacting with the integrins alpha5-beta1, alpha4-beta1 and alpha9-beta1 and has been shown to promote angiogenesis, lymphangiogenesis and metastasis of colorectal tumors. In transformed or tumor-derived cells the EDA segment is about 10-times higher than in fibronectin from normal fibroblasts and it may therefore be a significant marker for malignancy.

Purification: Puified

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant Extra Domain A (EDA) region of human cellular fibronectin.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile liquid. In PBS.

Preservative: Without preservative

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Target Details for Fibronectin

Target: Fibronectin