IFIH1 antibody

Catalog No. ABIN7566427

This Rabbit Polyclonal antibody specifically detects IFIH1 in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for IFIH1 antibody (ABIN7566427)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFIH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IFIH1 Antibody

Purpose: anti-MDA5 (mouse), pAb (AL180)

Characteristics:

Polyclonal Antibody. Recognizes mouse MDA5. Source: Rabbit. Applications: IHC, WB. Liquid. In PBS containing 0.02 % sodium azide. RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. MDA5, upon interaction with intracellular dsRNA (produced during viral replication), triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappaB, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). MDA5 is widely expressed at low levels. The expression is detected at higher levels in placenta, pancreas and spleen and barely in brain, testis and lung. Genetic variation in MDA5 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy.

RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. MDA5, upon interaction with intracellular dsRNA (produced during viral replication), triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappaB, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). MDA5 is widely expressed at low levels. The expression is detected at higher levels in placenta, pancreas and spleen and barely in brain, testis and lung. Genetic variation in MDA5 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy.

Purification: Puified

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse MDA5 (aa 2-208).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Target Details for IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: MDA5

UniProt: Q8R5F7

Pathways: Activation of Innate immune Response