Recombinant SARS-CoV-2 Spike S1 antibody

Catalog No. ABIN7566479

The Human Monoclonal anti-SARS-CoV-2 Spike S1 antibody has been validated for ELISA, WB and Func. It is suitable to detect SARS-CoV-2 Spike S1 in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for Recombinant SARS-CoV-2 Spike S1 antibody (ABIN7566479)

Target: SARS-CoV-2 Spike S1

Antibody Type: Recombinant Antibody

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Human

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Spike S1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Functional Studies (Func)

Clone: Covi-1

Product Details anti-SARS-CoV-2 Spike S1 Antibody

Purpose: anti-SARS-CoV-2 Spike Protein S1 mAb (rec.) (blocking) (Covi-1) (preservative free)

Characteristics:

Recombinant Antibody. Recognizes and binds to recombinant SARS-CoV-2 Spike Protein S1. It blocks the interaction of SARS-CoV-2 Spike Protein S1 (RBD) to human ACE2. Detects a different epitope as anti-SARS-CoV-2 Spike Protein S1, mAb (rec.) (blocking) (Covi-2) (Prod. No. AG-27B-6006PF). Applications: ELISA, FUNC (Blocking), WB. Source: HEK 293 cells. Liquid. In PBS. Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells.

Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells.

Purification: Puified

Purity: >98 % (SDS-PAGE)

Endotoxin Level: <0.001EU/μg

Immunogen: Recombinant SARS-CoV-2 Spike Protein S1 receptor binding domain.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS.

Preservative: Without preservative

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Target Details for SARS-CoV-2 Spike S1

Target: SARS-CoV-2 Spike S1

Alternative Name: SARS-CoV-2 Spike Protein S1

UniProt: P0DTC2