NOS1 antibody (AA 51-150)

Catalog No. ABIN725585

The Rabbit Polyclonal anti-NOS1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect NOS1 in samples from Human, Rat and Mouse. There are 3+ publications available.

Quick Overview for NOS1 antibody (AA 51-150) (ABIN725585)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NOS1 Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NOS-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NOS1 antibody (ABIN725585)

Zhu, Zhao, Luo, Li: "Gastrointestinal dysfunction in a Parkinson's disease rat model and the changes of dopaminergic, nitric oxidergic, and cholinergic neurotransmitters in myenteric plexus." in: Journal of molecular neuroscience : MN, Vol. 47, Issue 1, pp. 15-25, (2012) (PubMed).

Zhang, Liao, Ju, Song, Liu: "Neurochemical plasticity of nitric oxide synthase isoforms in neurogenic detrusor overactivity after spinal cord injury." in: Neurochemical research, Vol. 36, Issue 10, pp. 1903-9, (2011) (PubMed).

Wang, Yan, Zhao, Wu, Zhou: "The diversity of nNOS gene expression in avulsion-injured spinal motoneurons among laboratory rodents." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 22, Issue 1, pp. 37-42, (2010) (PubMed).

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: nNos

Background:

Synonyms: NOS, bNOS, nNOS, IHPS1, N-NOS, NC-NOS, Nitric oxide synthase, brain, Constitutive NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, NOS1

Background: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Gene ID: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction