ENOS antibody (AA 1105-1202)

Catalog No. ABIN725690

The Rabbit Polyclonal anti-ENOS antibody has been validated for WB, ELISA, IF (cc), IF (p), FACS, IHC (p) and IHC (fro). It is suitable to detect ENOS in samples from Human, Rat and Mouse. There are 9+ publications available.

Quick Overview for ENOS antibody (AA 1105-1202) (ABIN725690)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ENOS Antibody

Binding Specificity: AA 1105-1202

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NOS-3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ENOS antibody (ABIN725690)

Wu, Chou, Huang, Lin, Chen: "Bubble CPAP Support after Discontinuation of Mechanical Ventilation Protects Rat Lungs with Ventilator-Induced Lung Injury." in: Respiration; international review of thoracic diseases, Vol. 91, Issue 2, pp. 171-9, (2016) (PubMed).

Prayitnaningsih, Sujuti, Effendi, Abdullah, Anandita, Yohana, Permatasari, Widodo: "Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats." in: International journal of ophthalmology, Vol. 9, Issue 11, pp. 1568-1577, (2016) (PubMed).

Hu, Yan, Wang, Meng, Pan: "Comparative analysis of preservation method and intermittent perfusion volume on the expression of endothelial and inflammatory markers by coronal artery and myocardium in porcine donor hearts." in: ASAIO journal (American Society for Artificial Internal Organs : 1992), Vol. 60, Issue 6, pp. 681-7, (2014) (PubMed).

Wen, He, Xing: "Erythropoietin promotes retinal angiogenesis in a mouse model." in: Molecular medicine reports, Vol. 10, Issue 6, pp. 2979-84, (2014) (PubMed).

Nour, Yang, Dai, Wang, Feng, Lila, Chachques, Wu: "Intrapulmonary shear stress enhancement: a new therapeutic approach in acute myocardial ischemia." in: International journal of cardiology, Vol. 168, Issue 4, pp. 4199-208, (2013) (PubMed).

Sun, Wang, Huang, Cao: "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT2A/1B receptors and ?1-adrenoceptors in spontaneously rat arteries." in: European journal of pharmacology, Vol. 699, Issue 1-3, pp. 241-9, (2013) (PubMed).

Li, Han, Guo, Li, Sang: "Oxidative stress, endothelial dysfunction and inflammatory response in rat heart to NO? inhalation exposure." in: Chemosphere, Vol. 82, Issue 11, pp. 1589-96, (2011) (PubMed).

Ji, Min, Liang, Chen, Pan, Bin, Chen, Wei, He, Gu, Zhang: "Construction of tissue-engineered corpus cavernosum with muscle-derived stem cells and transplantation in vivo." in: BJU international, Vol. 107, Issue 10, pp. 1638-46, (2011) (PubMed).

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: eNOS

Background:

Synonyms: eNOS, ECNOS, Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, NOS type III, NOSIII, NOS3

Background: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Gene ID: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling