Actin antibody

Catalog No. ABIN726050

The Rabbit Polyclonal anti-Actin antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and FACS. It is suitable to detect Actin in samples from Human, Rat, Mouse, Chicken, Dog, Cow, Pig and Guinea Pig. There are 8+ publications available.

Quick Overview for Actin antibody (ABIN726050)

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Dog, Cow, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Product Details anti-Actin Antibody

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Pig,Chicken,Cat,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human alpha Actin

Isotype: IgG

Application Details

Application Notes: WB(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Actin antibody (ABIN726050)

Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." in: PLoS ONE, Vol. 9, Issue 6, pp. e99802, (2014) (PubMed).

Wang, Liao: "Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue." in: BMC urology, Vol. 14, pp. 69, (2014) (PubMed).

Song, Yan, An, Hao, Guo, Zhang, Li, Li, Sun: "Potential contribution of SOCC to cerebral vasospasm after experimental subarachnoid hemorrhage in rats." in: Brain research, Vol. 1517, pp. 93-103, (2013) (PubMed).

Lv, Fan, Su: "Is a swine model of arteriovenous malformation suitable for human extracranial arteriovenous malformation? A preliminary study." in: Cardiovascular and interventional radiology, Vol. 36, Issue 5, pp. 1364-70, (2013) (PubMed).

Liu, Xue, Tang, Hou, Qi, Chen, Chen, Zhang, Chen, Xu: "A simple method for isolating and culturing the rat brain microvascular endothelial cells." in: Microvascular research, Vol. 90, pp. 199-205, (2013) (PubMed).

Han, Liu, Li: "Salvianolic acid B inhibits autophagy and protects starving cardiac myocytes." in: Acta pharmacologica Sinica, Vol. 32, Issue 1, pp. 38-44, (2011) (PubMed).

Xu, Chen, Huang, Varghese, Moorhead, Yan, Powis, Li, Ruan: "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." in: American journal of physiology. Renal physiology, Vol. 301, Issue 4, pp. F713-22, (2011) (PubMed).

Waladali, Luo, Li, Zheng, Hu: "17Beta-estradiol affects the proliferation and apoptosis of rat bladder neck smooth muscle cells by modulating cell cycle transition and related proteins." in: World journal of urology, Vol. 27, Issue 2, pp. 241-8, (2009) (PubMed).

Target Details for Actin

Target: Actin

Background:

Synonyms: Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Molecular Weight: 42kDa

Gene ID: 58