Uricase (Uaz) antibody

Catalog No. ABIN7581193

This Chicken Polyclonal antibody specifically detects Uricase in ELISA and WB. It exhibits reactivity toward Aspergillus flavus.

Quick Overview for Uricase (Uaz) antibody (ABIN7581193)

Target: Uricase (Uaz)

Reactivity: Aspergillus flavus

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Purpose: Aspergillus flavus uricase Antibody

Characteristics: Chicken Anti-Rasburicase Antibody, Chicken Anti-Aspergillus flavus uricase Antibody

Purification: Chicken Anti-Aspergillus flavus uricase Antibody has been IgY affinity purified using sepharose resins with immobilized Aspergillus flavus uricase.

Sterility: Sterile filtered

Immunogen:

Immunogen: Recombinant Aspergillus flavus uricase

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: Affinity purified chicken polyclonal Anti-Aspergillus flavus uricase antibodies have been tested for use in Western Blot and ELISA. This antibody recognizes structured uricase and will work in western blot when the protein are either denatured or in its native form. Although not tested, this antibody being affinity-purified, polyclonal raised using whole A. flavus uricase immunized to chicken could be useful in detection of uricases from Aspergillus spp that share sequence homologies more than 80 % . Specific conditions for reactivity should be optimized by the end user. Western Blot Dilution: User Optimized ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store vial at 2-8° C. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: Uricase (Uaz)

Background: Aspergillus flavus uricase (Rasburicase) is currently used for the treatment of gout and hyperuricemia occurring in tumor lysis syndrome. Rasburicase, sold under the brand name Elitek in the US and Fasturtec in the EU, is a medication that helps to clear uric acid from the blood. It is a recombinant version of urate oxidase, an enzyme that metabolizes uric acid to allantoin. Urate oxidase is known to be present in many mammals but does not naturally occur in humans. The concentration of uric acid in human serum is 1.5-6.0 mg/dl for women and 2.5-7.0 mg/dl for men. Any increase in the catabolism of purines or decrease in uric acid excretion leads to build up of uric acid in the form of monosodium urate crystals in joints or soft tissues. Moreover, hyperuricemia is also observed tumor lysis syndrome (TLS) and is caused by massive tumor cell lysis followed by catabolism of the nucleic acids to uric acid after chemotherapeutic treatment.

UniProt: Q00511