Recombinant Myc Tag antibody (AA 408-439)

Catalog No. ABIN7581766

This anti-Myc Tag antibody is a Mouse Monoclonal antibody detecting Myc Tag in WB, ELISA, IF and FACS. Suitable for Tag.

Quick Overview for Recombinant Myc Tag antibody (AA 408-439) (ABIN7581766)

Target: Myc Tag

Antibody Type: Recombinant Antibody

Reactivity: Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Animal-Free

Clone: TUN219-2C1 (9E10)

Product Details anti-Myc Tag Antibody

Binding Specificity: AA 408-439

Expression System: Phage display

Purpose: Mouse Anti-c-myc Antibody, animal-free mAb

Specificity: This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10

Cross-Reactivity (Details): No known cross reactivity. No binding to native c-Myc protein.

Characteristics: This antibody can be detected with anti-mouse Fc secondary antibodies.

Purification: Protein A purification

Immunogen: No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4,

Storage: -20 °C

Target Details for Myc Tag

Target: Myc Tag

Alternative Name: c-myc tag

Target Type: Tag

Background: Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.

Molecular Weight: 1.2 kDa