Western Blotting (WB), ELISA, Immunofluorescence (IF)
Purpose
Rabbit anti-HA-tag Antibody, animal-free mAb
Specificity
This is an antibody developed by antibody phage display technology using a human naive antibody gene library and recombinant HA-tag antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.
Cross-Reactivity (Details)
No known cross reactivity.
Characteristics
This antibody can be detected with anti-rabbit Fc secondary antibodies.
Purification
Protein A purification
Grade
Animal-Free
Immunogen
No immunization, animal-free antibody development. Antigen: synthetic HA peptide corresponding to HA-tag (Human influenza hemagglutinin), aa 98-106, YPYDVPDYA
WB, IP, IF, IC
Host: Mouse
Monoclonal
unconjugated
Application Notes
Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS, pH 7.4, 0,02 % ProClin300
Preservative
ProClin
Precaution of Use
This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.