Iba1 antibody (Intracellular)

Catalog No. ABIN7581823

This Guinea Pig Polyclonal antibody specifically detects Iba1 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for Iba1 antibody (Intracellular) (ABIN7581823)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Iba1 Antibody

Binding Specificity: AA 40-53, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to IBA1/AIF1

Sequence: (C)DEDLQSKLEA FKTK

Specificity: Intracellular

Predicted Reactivity: Mouse - 13 out of 14 amino acid residues identical,human - 11 out of 14 amino acid residues identical

Characteristics: Guinea Pig Anti-IBA1/AIF1 Antibody (ABIN7581823) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IBA1/AIF1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-IBA1/AIF1 Antibody (ABIN7042910, ABIN7044214 and ABIN7044215) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DEDLQSKLEAFKTK, corresponding to amino acid residues 40-53 of rat IBA1/AIF1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234692)

Blocking Peptide: (ABIN7234692)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Ionized calcium-binding adapter molecule 1, Allograft Inflammatory Factor 1, MRF-1, Microglia response factor,Allograft Inflammatory Factor-1 (AIF1) is a 17 kDa. cytoplasmic, calcium and actin binding scaffold protein, mainly expressed in immunocytes. AIF-1 is induced by cytokines such as interferon-γ (IFN-γ).It is encoded within the HLA class III genomic region by IBA1 gene, and its structure contains seven alpha helixes and three beta sheets. There are three other proteins identical to AIF1: Ionized Ca2+-Binding Adaptor 1, Microglia Response Factor-1, and Daintain1.The gene is highly expressed in testis, spleen, circulating macrophages and in the brain but weakly expressed in lung and kidney. Among brain cells, the Iba1 gene is strongly and specifically expressed in microglia and its expression is up-regulated following nerve injury, central nervous system ischemia, and several other brain diseases2.AIF1 influences the immune system at several key points and thus modulates inflammatory diseases. It increases the expression of inflammatory mediators such as cytokines, chemokines, inducible nitric oxide synthase (iNOS) and promotes inflammatory cell proliferation and migration and hence it is highly associated with autoimmune diseases3. In addition, its abundance is positively correlated with metabolic indicators, such as body mass index, triglycerides, and fasting plasma glucose levels. There is also evidence that AIF1 could be a marker for diabetic nephropathy when detected in serum. It is found in activated macrophages in the pancreatic islets, and has been shown to decrease insulin secretion, while simultaneously impairing glucose elimination4.

Gene ID: 29427

UniProt: P55009

Pathways: Smooth Muscle Cell Migration