Apelin Receptor antibody (Extracellular, N-Term)

Catalog No. ABIN7581824

The Rabbit Polyclonal anti-Apelin Receptor antibody is suitable to detect Apelin Receptor in samples from Rat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for Apelin Receptor antibody (Extracellular, N-Term) (ABIN7581824)

Target: Apelin Receptor (APLNR)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apelin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Apelin Receptor Antibody

Binding Specificity: AA 4-17, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Apelin Receptor

Sequence: DGYNYYGADN QSEC

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Apelin/APJ Receptor (extracellular) Antibody (ABIN7581824) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Apelin receptor from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:600

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Apelin Receptor

Target: Apelin Receptor (APLNR)

Alternative Name: APLNR

Background:

Synonyms: APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34

Description: The apelin receptor (APJ) is a member of the G-protein coupled receptor gene family, encoded by the APLNR gene. The apelin receptor and its endogenous ligand, apelin, have been suggested to act as key mediators of physiological responses to multiple homeostatic actions, including cardiovascular control, water balance, hypothalamic-pituitary-adrenal (HPA) axis regulation and metabolic homeostasis.Apelin receptor structure contains seven hydrophobic transmembrane domains, with several sites for phosphorylation by protein kinase A, and sites for palmitoylation and glycosylation. The N-terminal glycosylation is implicated in the receptor's expression, stability, correct folding of the protein and ligand binding. The C-terminal palmitoylation has been reported to play a role in membrane association. These modifications can influence the internalization, dimerization and ligand binding of the receptor. Human apelin receptor shares 92 % homology with mouse receptor and 90 % homology with rat receptor.Apelin expression can be detected in several brain regions including the human caudate nucleus, corpus callosum, hippocampus, substantia nigra, subthalamic nucleus, medulla and spinal cord. Apelin receptor is also expressed in the spleen, placenta and cardiovascular tissues and small amounts in the small intestine, lung, stomach, colonic mucosa and ovary.

Gene ID: 83518

UniProt: Q9JHG3