ATP1A1 antibody (Intracellular)

Catalog No. ABIN7581827

The Guinea Pig Polyclonal anti-ATP1A1 antibody has been validated for WB and IHC. It is suitable to detect ATP1A1 in samples from Rat.

Quick Overview for ATP1A1 antibody (Intracellular) (ABIN7581827)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATP1A1 Antibody

Binding Specificity: AA 7-20, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to Alpha 1 Na+/K+ ATPase

Sequence: (C)RDKYEPAAVS EHGD

Specificity: Intracellular, N-terminus

Predicted Reactivity: Mouse,dog,pig - identical,Human- 13 out of 14 amino acid residues identical

Characteristics: Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody (ABIN7581827) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Alpha 1 Na+/K+ ATPase Antibody (ABIN7043659, ABIN7044637 and ABIN7044638) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236890)

Blocking Peptide: (ABIN7236890)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATP1A1

Background: ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1,Na+/K+-ATPase, a membrane protein located on the plasma membrane of all animal cells, is involved in maintaining the Na+ and K+ gradients across the cell membrane1. It is ubiquitously expressed in neurons and helps maintain normal neuronal function2. The enzyme is composed of a catalytic α chain of 112 kDa. and α glycosylated β chain of 40-50 kDa. There are four isoforms of the α subunit (α1, α2, α3 and α4) and three isoforms of the β subunit (β1, β2 and β3) with different tissue distributions3. The N-terminus is located on the cytoplasmic side of the membrane. There are four transmembrane segments in the first third of the polypeptide chain, a large cytoplasmic loop (LCL) comprising the second third of the chain and four to six transmembrane segments in the C-terminal third of the protein with the C-terminus also on the cytoplasmic side of the membrane4. Na+/K+-ATPase abnormalities have been reported to be involved in several neurological diseases such as seizures, bipolar disorder, spongiform encephalopathy, and Alzheimer's disease. Na+/K+-ATPase may also have implications in behavioral defects5.

Gene ID: 24211

UniProt: P06685

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process