BST1 antibody (Extracellular)

Catalog No. ABIN7581832

This Rabbit Polyclonal antibody specifically detects BST1 in WB, IHC, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for BST1 antibody (Extracellular) (ABIN7581832)

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-BST1 Antibody

Binding Specificity: AA 129-144, Extracellular

Purpose: A Rabbit Polyclonal antibody to C3aR1 (extracellular)

Sequence: (C)KVGDFLSW(S)RQENASG

Specificity: Extracellular, N-terminus.

Predicted Reactivity: Mouse,Rat - 15 out of 16 amino acid residues identical Not recommended for BST1 from human samples

Characteristics: Anti-CD157/BST1 (extracellular) Antibody (ABIN7581832) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD157 from mouse and rat samples. The antibody is not recommended for work with human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KVGDFLSW(S)RQENASG, corresponding to amino acid residues 129 - 144 of mouse BST1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for BST1

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Alternative Name: BST1 

Background: Bone Marrow Stromal Antigen 1, BST-1, ADP-Ribosyl Cyclase 2, Cyclic ADP-ribose hydrolase 2, CADPr Hydrolase 2, CADPR2, LY-65,CD157, also known as bone marrow stromal cell antigen 1 (BST1), is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein with a dual function as a receptor and a β-NAD+-metabolizing ectoenzyme of the ADP-ribosyl cyclases gene family1.CD157 and its paralog CD38, catalyze the formation of cyclic ADP-ribose (cADPR) from NAD, a second messenger involved in intracellular calcium mobilization through ryanodine receptors, among other functions 1,2.As a receptor, CD157 interacts with β1 and β2 integrins (CD29 and CD18 respectively) to form a multimolecular complex that mediates intracellular signaling through the FAK/Src pathway 1-3. Known ligands for CD157 include extracellular matrix components like fibronectin, fibrinogen, laminin-1 and type 1 collagen 2,3.As it name suggests, CD157 was first identified in bone marrow-derived cells, but it is also expressed in other immune system cells like neutrophils, T-cells, pre B-cells and macrophages. CD157 has been demonstrated to be involved in both the innate and adaptive immune response with roles in B- and T-cell growth and the control of myeloid cells migration during inflammation 1.CD157 is considered a marker and therapeutic target for several hematological malignancies like acute myeloid leukemia (AML) as well as several solid tumors like ovarian and malignant mesothelioma 3,4,5.Outside the immune system, CD157 is expressed in vascular endothelium, intestine and brain among others. Interestingly, CD157, has been identified as a risk factor for neurodegenerative diseases, particularly Parkinson's disease 2,6. The exact role of CD157 in the nervous system is not clear, but it appears to have a role in neurogenesis and neural stem cells proliferation, as well as a role in neuroinflammation probably through its association with CD18 and CD11b 2,7.Finally, studies with CD157 knockout mice, reveal its involvement in depression-like behaviors, social avoidance and anxiety disorders 2,8.

Gene ID: 12182

UniProt: Q64277