CARTPT antibody (Secreted)

Catalog No. ABIN7581841

This Rabbit Polyclonal antibody specifically detects CARTPT in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for CARTPT antibody (Secreted) (ABIN7581841)

Target: CARTPT (CART Prepropeptide (CARTPT))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARTPT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CARTPT Antibody

Binding Specificity: AA 95-108, Secreted

Purpose: A Rabbit Polyclonal antibody to CART peptide

Sequence: CDAGEQ(S)AVRKGAR

Specificity: Secreted

Cross-Reactivity (Details): The antibody will recognize the precursor (proCART) and both active CART peptides: CART (55-102) and CART (62-102). The human counterpart called CART (42-89) is identical to CART (55-102).

Predicted Reactivity: Mouse,rat,human - 13 out of 14 amino acid residues identical

Characteristics: Anti-CART Antibody (ABIN7581841) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CART peptide from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CDAGEQ(S)AVRKGAR, corresponding to amino acid residues 95 - 108 of mouse CARTPT

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CARTPT

Target: CARTPT (CART Prepropeptide (CARTPT))

Alternative Name: CARTPT

Background: Cocaine-And Amphetamine-Regulated Transcript Protein, CARTPT, CART Prepropeptide,CART peptide, known by the name CARTPT - Cocaine-And Amphetamine-Regulated Transcript Protein. CART peptide is a satiety factor closely associated with the actions of leptin and neuropeptide Y. This anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y (NPY) and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro1.CART peptide is a widely distributed neurotransmitter expressed in the central nervous systems, with a role in reward and reinforcement, feeding, development, sensory processing, stress, and endocrine control. Expression of CART peptide dominates in the mesocorticolimbic dopaminergic (DA) system that extends from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) and includes other limbic areas (amygdala, hippocampus, and frontal cortex), and is also widely distributed in the central nervous system (CNS)2.CART peptide exerted inhibitory effect on psychostimulant-enhanced dopamine receptors signaling, Ca2+/calmodulin-dependent kinase signaling and crucial transcription factors expression. Besides modulation of dopamine receptors-related pathways, CART peptide also exhibited elaborated interactions with other neurotransmitter receptors, such as glutamate receptors and γ-aminobutyric acid receptors, which further account for attribution of CART peptide to inhibition of psychostimulant-potentiated locomotor activity. CART peptide has been shown to have anxiolytic functions on the aversive mood and uncontrolled drug-seeking behaviors following drug withdrawal. Moreover, microinjection of CART peptide has been shown to have an anti-depressant effect, which suggests its potential utility in the mood regulation and avoidance of depression-like behaviors3.

Gene ID: 27220

UniProt: P56388

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour