FLRT3 antibody (Extracellular)

Catalog No. ABIN7581871

This Rabbit Polyclonal antibody specifically detects FLRT3 in WB, IHC, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for FLRT3 antibody (Extracellular) (ABIN7581871)

Target: FLRT3 (Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLRT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-FLRT3 Antibody

Binding Specificity: AA 387-401, Extracellular

Purpose: A Rabbit Polyclonal antibody to FLRT3 (extracellular)

Sequence: (C)KQPDIKNPKL TKDQR

Specificity: Extracellular, N-term.

Predicted Reactivity: Mouse - 14 out of 15 amino acids identical,Human - 13 out of 15 amino acids identical

Characteristics: Anti-FLRT3 (extracellular) Antibody (ABIN7581871) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize FLRT3 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KQPDIKNPKLTKDQR, corresponding to amino acid residues 387 - 401 of rat FLRT3 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FLRT3

Target: FLRT3 (Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3))

Alternative Name: FLRT3 

Background: Fibronectin Leucine Rich Transmembrane Protein 3, Leucine-rich repeat transmembrane protein FLRT3,The leucine-rich repeat (LRR) superfamily is a diverse protein family that consist of leucine-rich domains (LRR domains). The LRR domain is known to regulate protein-protein interaction or cell adhesion. A Large portion of LRR proteins mediate the differentiation and development of nervous tissues1.The fibronectin leucine-rich transmembrane 3 (FLRT3) protein play an important function during excitatory neuron development including neuron migration, axon guidance, or synapse formation2. FLRT3 gene is encoding single pass transmembrane domain with one extracellular LRR domain and a juxtamembrane fibronectin type 3 (FNIII) module. It was found to be expressed in kidney, brain, pancreases, skeletal muscle, lungs, liver placenta and heart3.FLRT3 display a unique multifunctional property acting as chemorepellent by binding to the Netrin receptor Unc5D and regulating radial migration of subventricular progenitors. In addition, it acts as homophilic adhesion molecule regulating the tangential dispersion of migrating neuron progenitors in the developing cortex4. FLRT3 knock out in mice leads to a variety of malformations, like disorganized basement membrane, failure of embryonic turning and ventral body closure, cardiac bifida and asymmetric development of headfolds5.

Gene ID: 366205

UniProt: B1H234