GAP43 antibody (C-Term)

Catalog No. ABIN7886621

The Guinea Pig Polyclonal anti-GAP43 antibody (ABIN7886621) specifically detects GAP43 in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for GAP43 antibody (C-Term) (ABIN7886621)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GAP43 Antibody

Binding Specificity: AA 205-218, C-Term

Purpose: A Guinea Pig Polyclonal Antibody to GAP43

Sequence: (C)KPKESARQDE GKED

Predicted Reactivity: Mouse - identical, human - 13 out of 14 amino acid residues identical

Characteristics: Guinea Pig Anti-GAP43 Antibody (ABIN7581879) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-GAP43 Antibody (ABIN7043263, ABIN7044662 and ABIN7044663) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235735)

Blocking Peptide: (ABIN7235735)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background:

Synonyms: Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Description: Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Gene ID: 29423

UniProt: P07936