GPR143 antibody (Intracellular)

Catalog No. ABIN7581885

This anti-GPR143 antibody is a Rabbit Polyclonal antibody detecting GPR143 in WB and IHC. Suitable for Mouse.

Quick Overview for GPR143 antibody (Intracellular) (ABIN7581885)

Target: GPR143 (G Protein-Coupled Receptor 143 (GPR143))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR143 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GPR143 Antibody

Binding Specificity: AA 225-239, Intracellular

Purpose: A Rabbit Polyclonal antibody to GPR143

Sequence: (C)KGRKGVYTEN ERLMG

Specificity: Intracellular, 3rd loop.

Predicted Reactivity: Rat- identical, human - 12 out of 15 amino acid residues identical

Characteristics: Anti-GPR143 Antibody (ABIN7581885) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR143 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KGRKGVYTENERLMG, corresponding to amino acid residues 225 - 239 of mouse GPR143

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400-1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR143

Target: GPR143 (G Protein-Coupled Receptor 143 (GPR143))

Alternative Name: GPR143

Background: G-Protein Coupled Receptor 143, Ocular Albinism Type 1 Protein, OA1,G-protein coupled receptor 143, GPR143, also known as ocular albinism type 1 protein, OA1, is an orphan GPCR expressed intracellularly in pigment-producing cells on the melanosomal membrane and serves to regulate melanosome size and mediate protein transport to the melanosome via the endolysosomal system.1GPR143 is encoded by the OA1 gene, composed of 404 amino acids with 7 transmembrane helices, and associates with several Gα and Gβ subunits, as well β-Arrestin. Potential ligands for GPR143 include L-3,4-dihydroxyphenylalanine (L-DOPA) and dopamine. High dose L-DOPA treatment leads to intracellular Ca2+ influx and β-Arrestin recruitment. GPR143 has two sorting signals, a dileucine motif in ICL3 and a tryptophan-glutamic acid doublet in the C-terminal tail, which are necessary for lysosomal and melanosomal localization. Functional components of endosomal sorting complexes required for transport (ESCRT) are essential for intracellular sorting and ubiquitination. Due to the unique localization of GPR143 within the cell and its topological orientation, ligands are likely required to bind from the organelle lumen.1GPR143 is largely expressed on the melanosomal membrane of melanocytes, the pigment producing cells that are responsible for producing melanin in the skin and eyes. Melanin is a pigment that is crucial for the protection of skin and eyes from damaging UV light, as well as for the normal development of the optic system and eye function. Mutations in the OA1 gene and its subsequent protein products lead to ocular albinism due to melanosome malformation. Albinism is a pigment disorder characterized by decreased melanin in affected tissues, which leads to adverse effects including foveal hypoplasia and chiasmal misrouting.1,3GPR143 pathophysiology is also implicated in various degenerative diseases, including macular degeneration and Parkinson's disease, as well as playing a possible role in cancer susceptibility and progression.1,2

Gene ID: 18241

UniProt: P70259