Granulin antibody (Secreted)

Catalog No. ABIN7581896

The Rabbit Polyclonal anti-Granulin antibody has been validated for WB and IHC. It is suitable to detect Granulin in samples from Mouse.

Quick Overview for Granulin antibody (Secreted) (ABIN7581896)

Target: Granulin (GRN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Granulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Granulin Antibody

Binding Specificity: AA 252-267, Secreted

Purpose: A Rabbit Polyclonal antibody to Progranulin

Sequence: CDLIQSK(S)LSKNYTTD

Specificity: Secreted

Predicted Reactivity: Mouse - 15 out of 16 amino acid identical, Human- 14 out of 16 amino acid identical, Rat- 13 out of 16 amino acid identical

Characteristics: Anti-Progranulin Antibody (ABIN7581896) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Progranulin from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CDLIQSK(S)LSKNYTTD, corresponding to amino acid residues 252 - 267 of mouse GRN

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Granulin

Target: Granulin (GRN)

Alternative Name: GRN

Background: Granulin Precursor, PGRN, Epithelin Precursor, Glycoprotein 88, GP88, PC Cell-Derived Growth Factor, PCDGF, GRN,The Progranulin (PGRN) protein is encoded by the GRN gene composed of 13 exons, although exon 1 is non-coding. PGRN is a secreted glycoprotein with growth factor like properties, consisting in 7.5 tandem repeats of a 12-cysteine module called granulin or epithelin domain separated by linker regions. The 12-cysteine motif forms six disulfide bridges with the peptide backbone adopting a unique three-dimensional structure of a parallel stack of beta hairpin1,2.PGRN is synthesized as a 68.5 kDa protein that is N-glycosylated to become a mature secreted 88 kDa protein3. Secreted PGRN is cleaved into 6 kDa granulin peptides, each composed of cysteine repeat motifs. Cleavage of PGRN occurs at the intra-linker spacer regions by several proteases including elastase4, proteinase5, matrix metalloproteinase-146 and ADAM metallopeptidase with thrombospondin type 1 motif7. PGRN cleavage is inhibited by secretory leukocyte protease inhibitor (SLPI)4 and high-density lipoprotein (HDL)/Apolipoprotein A-I complex8.PGRN is expressed in several tissues showing a prominent expression in epithelial, immune and, neuronal cells9. In particular, a strong expression of PGRN mRNA was found in highly proliferative epithelia including those of the intestinal crypt, skin, and reproductive tracts9. In contrast, PGRN shows low expression in less proliferative and highly differentiated cells such as those of alveolar lung and renal tube epithelia9.PGRN shows high expression in peripheral immune system tissues such as spleen and lymph nodes9 and is also highly expressed in the central nervous system (CNS) with expression in both neurons and neuroinflammatory cells. PGRN has been detected in neurons of the neocortex, hippocampus, arcuate nucleus and ventromedial hypothalamus, Purkinje cells of the cerebellum and in motor neurons9. In the CNS, PGRN can function as neurotrophic growth factor increasing cell viability10 and neurite outgrowth [11]. PGRN knockdown in rat hippocampal neurons results in decreased neuronal arborization and length and synaptic densities12 showing its role in neuronal morphology and establishing interneuron connections.Several proteins have been identified as receptors for GPRN, including Sortilin, which is considered the major regulator of GPRN in the brain, as well as, EphA2, LRP1, Notch and more13.Loss of function mutations in the GRN gene, cause a range of neurodegenerative disorders including frontotemporal dementia (FTD), amyotrophic lateral sclerosis (ALS), Alzheimer's and Parkinson's disease 13.

Gene ID: 14824

UniProt: P28798