HTR2C antibody (Extracellular)

Catalog No. ABIN7581899

The Rabbit Polyclonal anti-HTR2C antibody has been validated for WB and IHC. It is suitable to detect HTR2C in samples from Rat.

Quick Overview for HTR2C antibody (Extracellular) (ABIN7581899)

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR2C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HTR2C Antibody

Binding Specificity: AA 243-256, Extracellular

Purpose: A Rabbit Polyclonal antibody to 5HT2C Receptor (HTR2C)

Sequence: (C)RRQTLMLLRG HTEE

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse- identical, human - 12 out of 14 amino acid residues identical

Characteristics: Anti-5HT2C Receptor (HTR2C) Antibody (ABIN7581899) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5HT2C from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RRQTLMLLRGHTEE, corresponding to amino acid residues 243 -256 of rat HTR2C

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HTR2C

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Alternative Name: HTR2C

Background: 5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C,5-hydroxytryptamine receptor 2C, 5HT2C/5HT2CR, also known as serotonin receptor 2C, is a transmembrane (TM) receptor subtype in the 5-HT2R family of class A G-protein coupled receptors (GPCRs). 5HT2CR is encoded by the HTR2C gene and mediates various physiological functions, including disease-related pathways and behaviors due to its effects on the central nervous system (CNS).1,2The 5HT2C receptor is composed of seven TM helices (TM I - VII), three extracellular (ECL 1-3) and three intracellular loops (ICL 1-3), an intracellular carboxy-terminus, and an extracellular amino-terminus. Activation of 5HT2CR through 5-HT binding to Gαq/11 triggers G protein-dependent signaling that activates phospholipase Cβ (PLCβ) and leads to phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis to produce inositol-1,4,5-trisphosphate (IP3), accumulation of the downstream IP3 metabolite inositol monophosphate (IP1), and diacylglycerol (DAG), resulting in increased levels of intracellular mobilization of Ca2+ and IP1.1Activation of 5HT2CR can regulate ion channels and transport processes, as well as activate other downstream effectors including phospholipase A2 (PLA2), phospholipase D (PLD), cyclic nucleotides, and extracellular signal-regulated kinases (ERK1/2). Stimulation of 5HT2CR can activate cytosolic PLA2 to produce free arachidonic acid and its metabolites. Depending on the ligand, agonist signaling bias can activate either PLCβ or PLA2. Activation of 5HT2CR leads to protein kinase C (PKC) activation and downstream stimulation of the mitogen-activated protein kinase cascade, resulting in ERK1/2 phosphorylation. 5HT2CR has been shown to couple ERK1/2 through a PLD- and PKC-dependent pathway, potentially with Gα12/13 proteins.15HT2CR has been shown to interact with proteins including PSD-95/disk large/zonula occludens domain-containing proteins, calmodulin, β-arrestins, and phosphatase and tensin homolog, which all modulate receptor kinetics. β-arrestins play a significant role in regulating the functional activity of 5HT2CR by controlling desensitization and resensitization processes. Agonist-dependent desensitization of 5HT2CR involves phosphorylation by G-protein receptor kinase 2 (GRK2), binding of β-arrestin, and uncoupling of the receptor from G protein resulting in subsequent internalization into endosomes. Resensitization and recycling back to the plasma membrane occur upon dephosphorylation.1The expression and function of 5HT2CR play a primary role in the CNS. Serotonergic cell bodies in the midbrain dorsal raphe nuclei project to various brain regions, including the ventral tegmental area (VTA), nucleus accumbens (NAc), and prefrontal cortex (PFC), which are associated with reward and executive function. 5HT2CR is expressed postsynaptically in various neuronal cell types that use neurotransmitters such as acetylcholine, dopamine, and γ-aminobutyric acid (GABA), with a significant outcome of 5HT2CR stimulation being the modulation of dopamine neuronal function.15HT2CR plays a crucial role in regulating several physiological brain functions such as food intake, anxiety, stress response, motoneuron activity, and sleep. Dysregulation of 5HT2CR is observed in conditions such as depression, schizophrenia, suicidal behavior, spinal cord injury, and obesity.2

Gene ID: 25187

UniProt: P08909

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour