NMNAT2 antibody (Intracellular)

Catalog No. ABIN7581919

This Rabbit Polyclonal antibody specifically detects NMNAT2 in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for NMNAT2 antibody (Intracellular) (ABIN7581919)

Target: NMNAT2 (Nicotinamide Nucleotide Adenylyltransferase 2 (NMNAT2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMNAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NMNAT2 Antibody

Binding Specificity: AA 131-146, Intracellular

Purpose: A Rabbit Polyclonal Antibody to NMNAT2

Sequence: (C)HENTQPIYQN SNVPTK

Specificity: Intracellular.

Predicted Reactivity: Rat - identical,Human12,16 identical.

Characteristics: Anti-NMNAT2 Antibody (ABIN7581919) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NMNAT2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HENTQPIYQNSNVPTK, corresponding to amino acid residues 131 - 146 of mouse NMNAT2

Isotype: IgG

Application Details

Application Notes: Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NMNAT2

Target: NMNAT2 (Nicotinamide Nucleotide Adenylyltransferase 2 (NMNAT2))

Alternative Name: NMNAT2 

Background: Nicotinamide mononucleotide adenylyltransferase 2, Nicotinamide Nucleotide Adenylyltransferase 2, Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 2, NMN adenylyltransferase 2,Nicotinamide mononucleotide adenylyltransferase 2, also referred to as NMNAT2, belongs to the NMNAT family of enzymes responsible for NAD+ biosynthesis. This family catalyzes the final step in NAD+ production, converting nicotinamide mononucleotide (NMN) and ATP to NAD+. NMNAT2 is predominantly localized in the cytoplasm and Golgi apparatus, distinguishing it from NMNAT1 (nuclear) and NMNAT3 (mitochondrial) counterparts. Structurally, NMNATs share conserved ATP-binding motifs crucial for their enzymatic functions1-3. In addition to its enzymatic role, NMNAT2 also functions as a chaperone, mitigating protein aggregation under proteotoxic stress. Its enzymatic and chaperone activities are recruited in a context-dependent manner, protecting against excitotoxicity and proteotoxic stress, respectively. This dual functionality highlights its significance in cellular stress responses5. NMNAT2 plays a critical role in cellular energy metabolism by maintaining NAD+ pools, essential for redox reactions and the activity of sirtuins and PARPs, key regulators of gene expression and stress responses. NMNAT2's cytoplasmic localization facilitates its involvement in glucose metabolism and the regulation of compartmentalized NAD+ availability, which is crucial for processes like adipogenesis4. In neurons, NMNAT2 contributes to axonal protection and integrity by modulating NAD+-dependent repair pathways, highlighting its neuroprotective roles5. NMNAT2 protein levels are tightly regulated post-transcriptionally, with a short half-life necessitating constant replenishment, and its expression is induced by glucose metabolism3. Tissue-specific expression studies reveal that NMNAT2 is highly abundant in the brain, heart, and skeletal muscle, reflecting its importance in energy-demanding tissues. At the cellular level, its expression is notable in neurons, cardiomyocytes, and other metabolically active cells3-5. Clinically, reduced NMNAT2 levels have been observed in neurodegenerative diseases like Alzheimer's, where its decline precedes neurodegeneration, underscoring its potential as a therapeutic target. Dysregulated NMNAT2 activity has also been implicated in cardiac hypertrophy, demonstrating its broad relevance in disease pathophysiology1,3,5.

Gene ID: 226518

UniProt: Q8BNJ3