Neuregulin 1 antibody (Extracellular)

Catalog No. ABIN7581925

This Rabbit Polyclonal antibody specifically detects Neuregulin 1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Neuregulin 1 antibody (Extracellular) (ABIN7581925)

Target: Neuregulin 1 (NRG1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuregulin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Neuregulin 1 Antibody

Binding Specificity: AA 101-113, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Neuregulin-1 Type III

Sequence: (C)DKIFEYDSPT HLD

Specificity: Extracellular

Cross-Reactivity (Details): This antibody will recognize Neuregulin-1 Type III. It will not recognize Neuregulin-1 Type I, Type II and Type IV.

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-NRG1 Type III (extracellular) Antibody (ABIN7581925) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NRG1 Type III from rat, human and mouse samples. The antibody is specific for NRG1 Type III and does not recognize other Neuregulin-1 isoforms.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DKIFEYDSPTHLD, corresponding to amino acid residues 101-113 of human Neuregulin-1 Type III

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neuregulin 1

Target: Neuregulin 1 (NRG1)

Alternative Name: NRG1

Background: Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF,Neuregulin-1 (NRG1) is a member of a subfamily of the epidermal growth factor family and plays an important role in the development of the peripheral nervous system. Through alternative RNA splicing and promoter usage, the NRG1 gene encodes an entire family of more than 15 transmembrane and secreted isoforms. Neuregulin-1 acts as an important regulator in the survival of Schwann cell precursors, Schwann cell proliferation, motility, axon ensheathment and myelination.Neuregulin-1 can be divided into different according to the N-terminal sequence. Neuregulin-1 Type III, a transmembrane protein, contains an epidermal growth factor-like domain, which alone is adequate to bind and activate ErbB receptor tyrosine kinases. Type III also contains a cysteine-rich domain called "cysteine-rich domain-neuregulins," that requires a proteolytic cleavage for full activity1,2.Knockout studies show that type III is the key isoform required for schwann cell generation. These studies also confirmed that type III isoform is necessary for promoting myelination and to regulate myelin thickness1,2. Several different studies also showed that NRG1 Type III isoform is also an important regulator of cholinergic signaling. Neuregulin-1 Type III regulates the expression of AChR genes and targeting of nAChRs to the neuronal cell surface. This isoform in particular is a critical regulator of presynaptic targeting of α7-containing nAChRs3.

Gene ID: 3084

UniProt: Q02297

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation