Neurotrophin 3 antibody (AA 89-103)

Catalog No. ABIN7581927

This anti-Neurotrophin 3 antibody is a Rabbit Polyclonal antibody detecting Neurotrophin 3 in WB, IHC and IF. Suitable for Human.

Quick Overview for Neurotrophin 3 antibody (AA 89-103) (ABIN7581927)

Target: Neurotrophin 3 (NTF3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurotrophin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Neurotrophin 3 Antibody

Binding Specificity: AA 89-103

Purpose: A Rabbit Polyclonal Antibody to proNT-3

Sequence: (C)DTELLRQQRR YNSPR

Specificity: Pro-domain of the NT-3 protein

Cross-Reactivity (Details): The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.

Predicted Reactivity: Rat,mouse - identical

Characteristics: Highly specific antibody directed against an epitope of human pro. Anti-pro Antibody (ABIN7581927) can be used in western blot and immunohistochemistry applications. It has been designed to recognize pro from rat, mouse and human samples. The antibody does not cross reacts with mature , pro- and mature NGF or pro- and mature BDNF.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:300-1:600

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: NTF3

Background: Pro-Neurotrophin 3, proNTF3,Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins. These are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomain was thought to assist in the correct folding of the mature protein and the sorting of the neurotrophin into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions. Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions.It remains to be established whether proNT-3 has different biological functions than the mature NT-3 neurotrophin.

Gene ID: 4908

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway