PANX1 antibody (Intracellular)

Catalog No. ABIN7581943

This Guinea Pig Polyclonal antibody specifically detects PANX1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for PANX1 antibody (Intracellular) (ABIN7581943)

Target: PANX1 (Pannexin 1 (PANX1))

Reactivity: Human

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This PANX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PANX1 Antibody

Binding Specificity: AA 18-31, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to Pannexin 1

Sequence: (C)KEPTEPKFKG LRLE

Specificity: Intracellular, N-terminus

Predicted Reactivity: Rat,mouse - identical

Characteristics: Guinea Pig Anti-Pannexin 1 Antibody (ABIN7581943) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Pannexin 1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Pannexin 1 Antibody (ABIN7043426, ABIN7044100 and ABIN7044101) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236221)

Blocking Peptide: (ABIN7236221)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PANX1

Target: PANX1 (Pannexin 1 (PANX1))

Alternative Name: PANX1

Background: PANX1, PX1, MRS1, UNQ2529,Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.Proteins involved in gap junction formation are composed of four transmembrane domains, two extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9. Pannexin-1 was shown to interact with NMDA receptors in epilepsy, ischemia, pain and other pathologies10.

Gene ID: 25107

UniProt: Q96RD7