SCN2B antibody (Intracellular)

Catalog No. ABIN7581959

The Rabbit Polyclonal anti-SCN2B antibody is suitable to detect SCN2B in samples from Rat. It has been validated for WB, IHC and IF.

Quick Overview for SCN2B antibody (Intracellular) (ABIN7581959)

Target: SCN2B (Sodium Channel, Voltage-Gated, Type II, beta (SCN2B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-SCN2B Antibody

Binding Specificity: AA 197-215, Intracellular

Purpose: A Rabbit Polyclonal Antibody to NaVβ2 Subunit

Sequence: (C)KTEEEGKTDG EGNAEDGAK

Specificity: Intracellular, C-terminus

Predicted Reactivity: Human - 17,19 amino acid residues identical

Characteristics: Anti-SCN2B (NaVβ2) Antibody (ABIN7581959) is a highly specific antibody directed an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaVβ2 subunit from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat beta2 subunit of voltage-gated Na+ channels

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN2B

Target: SCN2B (Sodium Channel, Voltage-Gated, Type II, beta (SCN2B))

Alternative Name: SCN2B

Background: Voltage-gated sodium channel subunit beta-2,Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.2,3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β-subunits (NaVβ-subunit).4The NaV β-subunit gene family consists of four members: β1 (SCN1B), β2 (SCN2B), β3 (SCN3B), and β4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus. They modulate channel gating, assembly, and cell surface expression in heterologous cell systems.5NaV β-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules.5In the adult central nervous system and heart, sodium channels are associated with β1- β4 subunits, whereas in adult skeletal muscle they are associated only with the β1 subunit.4,5 This association appears to be a late event in sodium channel biosynthesis.6Expression of β2-subunits increases in sensory neurons following nerve injury. β2 deficient mice develop less mechanical allodynia in comparison to wild type mice, suggesting a role for β2 subunit in pain sensation.7

Gene ID: 25349

UniProt: P54900