SLC6A1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7581982

This Guinea Pig Polyclonal antibody specifically detects SLC6A1 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for SLC6A1 antibody (2nd Extracellular Loop) (ABIN7581982)

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SLC6A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLC6A1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 194-206

Purpose: A Guinea Pig Polyclonal Antibody to GABA Transporter 1 (GAT-1) (extracellular)

Sequence: (C)ERNMHQMTDG LDK

Predicted Reactivity: Mouse,human - identical

Characteristics: Guinea Pig Anti-GABA Transporter 1 (extracellular) Antibody (ABIN7581982) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-GABA Transporter 1 (extracellular) Antibody (ABIN7043178, ABIN7044459 and ABIN7044460) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235465)

Blocking Peptide: (ABIN7235465)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SLC6A1

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Alternative Name: SLC6A1

Background:

Synonyms: GABATR, GABT1, GABATHG, Na+/Cl-- dependent GABA transporter 1, Solute Carrier Family 6 Member 1, SLC6A1

Description: γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1.GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4.GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7.

Gene ID: 79212

UniProt: P23978