TRHR antibody (Intracellular)

Catalog No. ABIN7581994

This Rabbit Polyclonal antibody specifically detects TRHR in WB, IF and IC. It exhibits reactivity toward Rat.

Quick Overview for TRHR antibody (Intracellular) (ABIN7581994)

Target: TRHR (Thyrotropin-Releasing Hormone Receptor (TRHR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRHR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-TRHR Antibody

Binding Specificity: AA 336-349, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Thyrotropin-Releasing Hormone Receptor 1

Sequence: CKQKPTEKAA NYS

Specificity: Intracellular, C-terminus

Predicted Reactivity: Mouse - identical, human -12,13 amino acid residues identical

Characteristics: Anti-TRHR1 Antibody (ABIN7581994) is a highly specific antibody directed against an epitope of the rat Thyrotropin-releasing hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize TRH-R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CKQKPTEKAANYS, corresponding to amino acid residues 336-349 of rat Thyrotropin-releasing hormone receptor 1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRHR

Target: TRHR (Thyrotropin-Releasing Hormone Receptor (TRHR))

Alternative Name: TRHR

Background: Thyrotropin-releasing hormone receptor 1, TRH1 receptor, TRHR, Thyroliberin receptor,Thyrotropin-releasing hormone (TRH) is a widely distributed tripeptide which acts as a hormone, a paracrine regulatory factor and a neurotransmitter/neuromodulator. It is expressed in many parts of the brain as well as in various peripheral tissues1,2.In the hypothalamus, TRH is secreted from the paraventricular nucleus (VPN) by TRH neurons prior to a post-translational processing from its precursor, pro-TRH. A key player in the regulation of the hypothalamus-pituitary-thyroid (HPT) axis, TRH is released into the hypophyseal portal circulation of the median eminence and reaches the pituitary, where it binds to TRH receptors, thereby stimulating the secretion of tyrothropin (thyroid-stimulating hormone, TSH). TSH, in turn, affects the thyroid to produce T4 which is subsequently converted into its biologically-active analog, T3 - a negative mediator of TRH. In the tanycytes, ependymal cells of glial origin located in close proximity to the VPN, T4 is converted to T3 by the enzyme deiodinase II (dio2) - a counterpart to deiodinase III (dio3) which conversely inactivates the above mentioned thyroid hormones, thus indirectly promoting the production of TRHs3,4.TRH receptor 1 (TRH-R1), the only identified TRH receptor in humans, is a G-protein coupled receptor (GPCR) abundant in the anterior pituitary, neuroendocrine system, autonomic nervous system and the visceral regions of the brain2,5. Like all members of GPCRs, the TRH receptor has seven transmembrane domains, and extracellular N-terminus and intracellular C-terminal tail. TRH receptor couples Gq upon activation which in turn leads to the activation of phospholipase C5.From a behavioral aspect, it was shown that TRH-coupled TRH-R1 increases cognitive arousal by directly exciting the sleep/wake system in the lateral hypothalamic area, and inhibits seizure activity in the hippocampus by facilitating GABA release. Conversely, its deficiency, as observed in mutant model mice, is linked to growth retardation, depression, and anxiety-like behavior1,6,7.

Gene ID: 25570

UniProt: Q01717