GPR142 antibody (Extracellular) (FITC)

Catalog No. ABIN7582032

This anti-GPR142 antibody is a Rabbit Polyclonal antibody detecting GPR142 in FACS and LCI. Suitable for Mouse.

Quick Overview for GPR142 antibody (Extracellular) (FITC) (ABIN7582032)

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR142 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR142 Antibody (FITC)

Binding Specificity: AA 291-304, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR142 (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)APVHRDWRVH LALD

Specificity: Extracellular, 3rd loop.

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-GPR142 (extracellular) Antibody (ABIN7237652, ABIN7316613 and ABIN7316614) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR142 from rat, mouse and human samples. Anti-GPR142 (extracellular)-FITC Antibody (ABIN7237652, ABIN7316613 and ABIN7316614-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235372)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR142

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Alternative Name: GPR142

Background: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2,G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33 % amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.

Gene ID: 217302

UniProt: Q7TQN9