GPR4 antibody (Extracellular) (FITC)

Catalog No. ABIN7582035

This anti-GPR4 antibody is a Rabbit Polyclonal antibody detecting GPR4 in FACS and LCI. Suitable for Rat.

Quick Overview for GPR4 antibody (Extracellular) (FITC) (ABIN7582035)

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR4 Antibody (FITC)

Binding Specificity: AA 162-177, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR4 (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)RDRYNHTF(S)FEKFPME

Specificity: 2nd extracellular loop

Predicted Reactivity: Rat,mouse,human - 15,16 amino acid residues identical

Characteristics: Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize GPR4 from mouse, rat and human samples. Anti-GPR4 (extracellular)-FITC Antibody (ABIN7043160, ABIN7044423 and ABIN7044424-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR4 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235415)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR4

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Alternative Name: GPR4

Background: Acid-sensing G-protein coupled receptor 4, GPR19,G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

Gene ID: 308408

UniProt: Q4KLH9