OLIG2 antibody

Catalog No. ABIN7595310

The Guinea Pig Polyclonal anti-OLIG2 antibody has been validated for WB, IHC and FACS. It is suitable to detect OLIG2 in samples from Mouse.

Quick Overview for OLIG2 antibody (ABIN7595310)

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivity: Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-OLIG2 Antibody

Purpose: Guinea pig antibody to Olig2

Specificity: Specific for Olig2.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: Whole serum

Immunogen: A synthetic peptide from mouse Olig2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Application Details

Application Notes: IHC WB Flow Cyt. A dilution of 1 : 500 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: Olig2

Background: Function: Required for oligodendrocyte and motor neuron specification in the spinal cord as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. Subcellular location: Nucleus. Cytoplasm Tissue specificity: Expressed in the brain in oligodendrocytes. Strongly expressed in oligodendrogliomas while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. Involvement in disease: A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14,21)(q11.2,q22) with TCRA.