NOS1 antibody

Catalog No. ABIN7595322

The Chicken Polyclonal anti-NOS1 antibody has been validated for WB and IHC. It is suitable to detect NOS1 in samples from Human.

Quick Overview for NOS1 antibody (ABIN7595322)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NOS1 Antibody

Purpose: Chicken antibody to nNOS

Specificity: Specific for nNOS

Cross-Reactivity: Human, Marmoset, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: Purified IgY

Immunogen: A synthetic peptide from human nNOS conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and shares 96% identity with rat's sequence.

Isotype: IgY

Application Details

Application Notes: IHC WB. A dilution of 1: 500 is recommended. The optimal dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term.

Expiry Date: 12 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: nNOS

Background: FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. SUBCELLULAR LOCATION: Cell membrane sarcolemma, Peripheral membrane protein. Cell projection dendritic spine. Note=In skeletal muscle it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons enriched in dendritic spines. TISSUE SPECIFICITY: Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis urethra prostate and skeletal muscle and coexists with the cerebellar nnos in the pelvic plexus bladder and liver and is detectable in the cerebellum.

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction