Hemagglutinin antibody

Catalog No. ABIN7595337

The Rabbit Polyclonal anti-Hemagglutinin antibody has been validated for ELISA, WB and IHC. It is suitable to detect Hemagglutinin in samples from Influenza A Virus.

Quick Overview for Hemagglutinin antibody (ABIN7595337)

Target: Hemagglutinin (HA)

Reactivity: Influenza A Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hemagglutinin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Hemagglutinin Antibody

Virus Strain: A/Singapore/1/1957

Purpose: Rabbit antibody to Influenza A virus HA (25...515)

Specificity: Specific for Influenza A virus HA.

Cross-Reactivity: Influenza A Virus

Purification: Purified IgG

Immunogen: A chimeric recombinant hemagglutinin from Influenza A virus (strain A/Singapore/1/1957 H2N2) containing regions 25-56 110-140 194-232 365-423 443-472 and 490-515 was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB ELISA. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. 

Expiry Date: 12 months

Target Details for Hemagglutinin

Target: Hemagglutinin (HA)

Alternative Name: Hemagglutinin

Target Type: Influenza Protein

Background: Hemagglutinin: Major glycoprotein comprises over 80 % of the envelope proteins present in virus particle. The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes allowing the virus to grow in other organs than lungs. FUNCTION: Binds to sialic acid-containing receptors on the cell surface bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2 releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.